The project focuses on development of the sequencing-by-synthesis principle, to make it well adapted for applications in the field of DNA diagnostics. Techniques based on the sequencing-by-synthesis principle, for faster, longer and more robust sequencing are studied. The goal is to allow sequencing of up to 500 bases and thereby perhaps outperform the Sanger method. The possibility to develop and utilize a mathematical model of the DNA sequencing reaction for faster optimization of the method is explored in collaboration with Professor Anders Lansner at the Department of Numerical Analysis and Computer Science, KTH. Reagents, chemistry and enzymatic activities suggested by the model will be analyzed under experimental conditions. Multiple infections have been a problem when genotyping by the Sanger method and also by the sequencing-by-synthesis principle (Pyrosequencing method), as double/multiple infections present in one specimen give rise to sequence signals from all available type(s). In this project, we address this problem by a novel multiple-primer DNA sequencing approach.
Biotechnology, Bioscience, Chemistry, Medical technology